Regulation of TRIM E3 Ligases and Cyclophilin A and the Impact on HIV-1 Replication and Pathogenesis

Ravesh Singh, Thumbi Ndung'u


An important part of the innate defense against viruses is the production of the type I interferons, IFN- ?, and IFN-?.  INF-1 induces members of the Tripartite interaction motif (TRIM) family of E3 ligases whose contribution to prevention of infection or control of HIV replication in vivo is not completely understood.  Cyclophilin A (CypA), a human protein that is incorporated into the HIV-1 virion increases viral infectivity by facilitating proper uncoating of HIV-1 capsid.  Here we investigated the association between interferon-1 (INF-?, and INF-?), MXA (a marker of type 1 interferons), with TRIM family E3 ligases,( huTRIM5?, TRIM11, TRIM 19, TRIM 22 and TRIM 36) and Cyclophilin A (CypA) during primary HIV-1 infection.