Mutation frequency in drug-susceptible clinical isolates of Mycobacterium tuberculosis under aerobic and anaerobic conditions

Lavania Joseph, Dr A.W Sturm

Abstract


Mycobacterium tuberculosis (Mtb) infections result in either active or latent disease.  Previous models of latency have indicated little or no capacity for growth and metabolic activity of Mtb, as opposed to active infection. The mutation frequency is supposed to be highest during active replication. Recent studies in macaques found a higher mutation frequency during latency as compared to active infection. The type of mutation suggests that these result from redox events. We will measure the mutation frequency by means of fluctuation analysis under aerobic and anaerobic incubation. The redox potential in the culture media will be measured during the full incubation period.