Production and evaluation of anti-pili monoclonal antibodies for the development of a potential point of care diagnostic test for Mycobacterium tuberculosis

Natasha Pillay, Manormoney Pillay

Abstract


 

Aims (objectives of study)

The aim of this study is to develop and evaluate

Mycobacterium tuberculosis anti-pili monoclonal antibodies for use in a point of care (POC) immunochromatographic test. The following objectives were identified to achieve the above aim:

1. To determine the presence of the

mtp gene in clinical strains of Mycobacterium tuberculosis, Mycobacteria other than tuberculosis (MOTTs) and other respiratory pathogens and commensals.

2. To clone and express recombinant pili protein

3. To use the recombinant protein to determine the prevalence of anti-pili antibodies in patients with different stages of TB infection and disease

4. To produce anti-pili monoclonal antibodies

5. To determine the sensitivity and specificity of the monoclonal anti-pili antibodies in detecting mycobacterial pili protein in clinical strains of

M. tuberculosis, MOTTs and respiratory pathogens and commensals

6. To determine the sensitivity and specificity of the monoclonal anti-pili antibodies in detecting mycobacterial pili protein in patients with active tuberculosis using ELISA.