The effect of Mycobacterium tuberculosis from patients with spinal TB and pulmonary TB in tissue culture systems

Muyalo Glen Dlamini, Prashini Moodley

Abstract


  1. 1.              Summary of the proposed research (restrict to 100 words)

 

Patients presenting with Pott’s disease to the Spinal Unit at King George V hospital will be recruited. Specimens will be collected from the infected lesions during surgery by an orthopaedic surgeon, Prof S Govender. Stored MTB isolates obtained from patients with pulmonary TB will be used as per BREC #274/09.

After decontamination specimens will be cultured in broth and 7H11 media for about 3 weeks. Cell lines (MH-S alveolar macrophage cell line, A549 alveolar epithelial cell line, Saos-2 osteoblastic cell line) will be prepared by culturing in the appropriate media. Cell lines will then be inoculated with MTB and the affinity and invasion capacity of MTB for each of these cell lines will be determined.