Development of Novel Reagents for Tuberculosis Detection

Nqobile Ngubane, Alexander Pym

Abstract


Mycobacterium tuberculosis is the causative agent of the infectious disease tuberculosis (TB).  The failure in controlling the spread of the disease can mainly be attributed to the inability to detect the disease state using quick, sensitive and affordable methods. In response to this unmet medical need, we propose to identify novel reagents for early detection of active TB. The objective is to isolate and characterise aptamers against M. tuberculosis secreted proteins and/or short peptides against the whole M. tuberculosis bacillus. To demonstrate their potential for use in diagnostics, the reagents will be tested for specificity on different M. tuberculosis clinical isolates