Impact of Gag p2/p7NC cleavage site polymorphisms on HIV-1 subtype C viral fitness

Serron Wilson, Michelle Gordon


Subtype C is the dominant HIV subtype in South Africa and has several distinct characteristics, which could be responsible for this. One such feature is the high degree of variation at certain Gag cleavage sites, in particular, the p2/p7NC cleavage site. Previous studies on the Gag cleavage site mutations indicate that variation at the sites may have a significant influence on the virus. This study aims to determine the phenotypic effect of the genotypic variability. Computational methods will be used to identify a range of variants which will then be generated and characterized in terms of Gag cleavage and the replication capacity