An Outbreak of Foodborne Salmonellosis in Rural KwaZulu-Natal, South Africa

Abraham Johannes Niehaus, Prashini Moodley

Abstract


Isolation, identification and susceptibility testing of isolates have already been completed during the initial outbreak investigation. Further work will include determining the minimum inhibitory concentrations of all isolates against ampicillin, ceftriaxone, cefuroxime, chloramphenicol, ciprofloxacin, meropenem, nalidixic acid and tetracycline; using the microbroth dilution method following CLSI guidelines. Morphological similarity of isolates will be investigated by comparing phenotypical characteristics including API 20E profiles, VITEK 2 results, antimicrobial susceptibility profiles (including disk diffusion, VITEK 2 and microbroth dilution results) and serotyping results. Genotypic relatedness will be assessed with pulsed-field gel electrophoresis using the CHEF-DRIII instrument.